Biology - Microscopy Practical

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Why is iodine solution added to onion cells?
оқуды бастаңыз
To stain the cells so they are easier to see.
Why is coverslip lowered at an angle?
оқуды бастаңыз
To prevent air bubbles from forming.
Which objective lens should you start with?
оқуды бастаңыз
The lowest power objective (usually 4x)
Which focusing knob should be used first?
оқуды бастаңыз
The coarse adjustment knob.
Which focusing knob is used for clear focus?
оқуды бастаңыз
The fine adjustment knob.
What should you do after finding cells under low power?
оқуды бастаңыз
Switch to a higher power lens and refocus using the fine adjustment knob.
How is total magnification calculated?
оқуды бастаңыз
Total magnification=eyepiece lens x objective lens
What should the biological drawings include?
оқуды бастаңыз
Labels and magnification.
What is the formula for calculating actual size?
оқуды бастаңыз
Actual size=image size/magnification
How many micrometres are in 1mm?
оқуды бастаңыз
1000 micrometres
Put the osmosis practical steps in order: Record final mass, Place in solutions, Cut potato cylinders, Blot dry, Record initial mass
оқуды бастаңыз
1. Cut potato cylinders 2. Record initial mass 3. Place in solutions 4. Blot dry 5. Record final mass
Put these microscope steps in order: Use fine focus, Place slide on stage, Choose low power lens, Use coarse focus, Switch to higher power
оқуды бастаңыз
1. Place slide on stage 2. Choose low power lens 3. Use coarse focus 4. Use fine focus 5. Switch to higher power

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